| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069616 |
| Chromosome: | chromosome 10 |
| Location: | 2417868 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435250 | NIC13 | (1 of 1) 6.3.5.1 - NAD(+) synthase (glutamine-hydrolyzing) / Nicotinamide adenine dinucleotide synthetase (glutamine); NAD+ synthase (glutamine-hydrolyzing) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCGCATGTCACGGCACGTACCCGCCAACCCATCCTCTTCACACGTCG |
| Internal bar code: | TATATGTTTCAGCTTTTTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1588 |
| LEAP-Seq percent confirming: | 92.2222 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGCGTTCATGTAACCGC |
| Suggested primer 2: | TTCCATTTGGCTGCACTTGC |