Insertion junction: CLIP2.069617_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.069617
Chromosome:chromosome 6
Location:1892760
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g264150 MAW11 Membrane-associated hydroxyproline-rich glycoprotein 11; (1 of 1) 2.1.1.53 - Putrescine N-methyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGACTTGACGCTGCTGGCGTTTTTCATCTGTGGATTTCAATCTGACT
Internal bar code:GGGACACCCTCGAATGTAGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1227
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAGTGACCTTTCGTCCGG
Suggested primer 2:GTAGGTTGTCGCTGGTGTGA