| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069642 |
| Chromosome: | chromosome 10 |
| Location: | 363182 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420450 | (1 of 2) PTHR18841:SF0 - PROTEIN C08G5.3, ISOFORM A-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACGTCTTTTCGCTCTGCGCCGGCTACAACTGGGCAGGTGGGTGCGGG |
| Internal bar code: | CTCTAAATACAGACTGTACGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 542 |
| LEAP-Seq percent confirming: | 46.6667 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACGATCTTCCACTTGCC |
| Suggested primer 2: | AGCTCAACCTGTCATCACCG |