| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069661 |
| Chromosome: | chromosome 9 |
| Location: | 2871100 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390700 | POB1 | Proteome of basal body 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCATATTGGGACGGAAGGAGTGTACAACATAGCATGTCAACGAGACAC |
| Internal bar code: | TAATTTGTTGAACCCATTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 35.7143 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCAGCAGTGTGGAGGG |
| Suggested primer 2: | TCACGTGAACGCATGAGGAA |