Insertion junction: CLIP2.069663_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.069663
Chromosome:chromosome 7
Location:3924683
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g339350 (1 of 1) K14050 - geranylgeranyl transferase type-2 subunit alpha [EC:2.5.1.60] (RABGGTA) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTTGAGTGTTCCGCTACAGGATAACTCATGGTCTTACATGTAGCATGC
Internal bar code:TCGCGGGCTCGGGGTATATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:865
LEAP-Seq percent confirming:64.1026
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGGAGCTCATCACTGGCT
Suggested primer 2:CTTCTGTCCCGCTGTCGTAG