| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069683 |
| Chromosome: | chromosome 6 |
| Location: | 4786873 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279700 | (1 of 1) PTHR15535:SF22 - PROTEIN RLIB | 3'UTR | |
| Cre06.g279750 | MAP1A | (1 of 1) PTHR10804//PTHR10804:SF13 - PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P // METHIONINE AMINOPEPTIDASE 1; Methionine aminopeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGATCGGACGAAGTGCTTCTGGGGTGTCTTGTATCGAGCAGCCCCAA |
| Internal bar code: | GGGTGTGCCAGAGTACATTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5061 |
| LEAP-Seq percent confirming: | 95.0617 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCAGTATTGTGTGGTCCC |
| Suggested primer 2: | TCGAACGCAACAGGGATTCA |