| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069694 |
| Chromosome: | chromosome 2 |
| Location: | 7471097 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392430 | (1 of 1) 1.1.1.62 - 17-beta-estradiol 17-dehydrogenase / Estrogen 17-oxidoreductase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCCCGATGGGCGCGTGTCGGTCCGATCGGGCCCAAGTGTGCTGGAGA |
| Internal bar code: | CGTGATTGTTAGTATCATCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3489 |
| LEAP-Seq percent confirming: | 85.3659 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCGTCCAAAGACCAAAC |
| Suggested primer 2: | GAGACGAGGAGCGAATGAGG |