| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069696 |
| Chromosome: | chromosome 12 |
| Location: | 5457188 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529800 | (1 of 31) IPR003590 - Leucine-rich repeat, ribonuclease inhibitor subtype | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAGATGCAGAGTGATGGTCGGCACAGCAGCCGTGGGCCCCGATGTCA |
| Internal bar code: | GGGGTGTTACTCCCTGGGAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2025 |
| LEAP-Seq percent confirming: | 98.3333 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCTCCGTGTTGACTGA |
| Suggested primer 2: | GGTGGTGGAGAAGGTGATGG |