| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069705 |
| Chromosome: | chromosome 8 |
| Location: | 128054 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358534 | (1 of 2) IPR000679//IPR001025 - Zinc finger, GATA-type // Bromo adjacent homology (BAH) domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTACGGGAGTCTCATGTGCGTAGAAAGCGATGACGCATATTTCTTGCAG |
| Internal bar code: | GCGCCTGTCTAAATATCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2501 |
| LEAP-Seq percent confirming: | 72.5 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGACTGAACAGTGGTCGA |
| Suggested primer 2: | ATGATCTGGACTGGCCTTGC |