| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069719 |
| Chromosome: | chromosome 6 |
| Location: | 843814 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255600 | EZY24,EZY1,EZY1A | Copy A in the EZY1 tandem gene cluster | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTAGTCTGCGGCCGCGGCAGGCCCAGGAGGAGTCTGGGTGACGAACT |
| Internal bar code: | GCATGTAATTTAGATAGTCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5095 |
| LEAP-Seq percent confirming: | 43.4783 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCCCCCAAAACCAAAG |
| Suggested primer 2: | GCCGCATTCACCATCACATC |