Insertion junction: CLIP2.069733_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.069733
Chromosome:chromosome 14
Location:878428
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre14.g613134 (1 of 11) 2.1.1.259 - [Fructose-bisphosphate aldolase]-lysine N-methyltransferase / (dimerizing)]-lysine 6-N-methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAATTCCTGCCCCTCCATCCCCCCCTCCTGCTCGCAGTCGCGCCCCGC
Internal bar code:CGGTTATGATATTTACTTCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:745
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CATACCACACGTCCCACCAA
Suggested primer 2:CCCGTGGATTTGGTGATGGA