| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069741 |
| Chromosome: | chromosome 12 |
| Location: | 4588675 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521700 | SSS6,SSS1A | Soluble starch synthase IA; (1 of 5) K00703 - starch synthase (E2.4.1.21, glgA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTCTTGTCTTGAGCGTGTGTGTACGAGCTCGGAATACTGTGTGCATGT |
| Internal bar code: | CGGGAGCATGGGCAATTGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1714 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACGTTTGATGACGGCTGA |
| Suggested primer 2: | GGAGAGCGTGATCGAGACTG |