| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069742 |
| Chromosome: | chromosome 3 |
| Location: | 7284219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207250 | GLN4 | (1 of 2) PTHR20852:SF50 - GLUTAMINE SYNTHETASE CYTOSOLIC ISOZYME 1-1-RELATED; Glutamine synthetase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACTTATAACCTCGAGCTTCTGCCAGGGCCAACCTAAGCGCGCGAGCAG |
| Internal bar code: | TCCGGTTATCGGAGCTGGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2446 |
| LEAP-Seq percent confirming: | 63.6364 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGCGTGAGAACATGAA |
| Suggested primer 2: | AAACAACCTGACCAATGCGC |