| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069766 |
| Chromosome: | chromosome 5 |
| Location: | 1253074 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241636 | (1 of 1) PTHR23042:SF72 - TRANSCRIPTION FACTOR BHLH104-RELATED | 5'UTR | |
| Cre05.g241637 | FAP32,IFT46 | Intraflagellar transport protein 46; (1 of 1) PTHR13376//PTHR13376:SF0 - UNCHARACTERIZED // INTRAFLAGELLAR TRANSPORT PROTEIN 46 HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCTGCTTGAGTCACCACGGATGGCGTGTGCCCCGCACAGGACCGTGA |
| Internal bar code: | GCTGGGCGATCCGGCAAGAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1490 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTGCCTCAACGCATGTTT |
| Suggested primer 2: | CGTCCGAGTCTGATAAGGCC |