| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.069788 |
| Chromosome: | chromosome 3 |
| Location: | 4582808 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176450 | (1 of 17) IPR029069 - HotDog domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCAGCACCCAAGAAGTGCTTGAAGGGCTTGCAACATCGGCAAGCACT |
| Internal bar code: | CATTGAGAGATGGCGACCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 1.75439 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTGCACAGACCTTGTTG |
| Suggested primer 2: | ACATCCGTAAACCGTCGCTT |