Insertion junction: CLIP2.069793_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.069793
Chromosome:chromosome 11
Location:1788703
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467750 5'UTR
Cre11.g467800 (1 of 1) PF06545 - Protein of unknown function (DUF1116) (DUF1116) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATCACGACTGATCGACACCTATATAGCAACCACTGGCCTCTGTAATTG
Internal bar code:TTTACTTCAGGGCGTCGAGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1762
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTACCGCTATAACCGCTG
Suggested primer 2:GATCCCAGTTGAGGCGACAA