| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069807 |
| Chromosome: | chromosome 12 |
| Location: | 2848683 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503200 | (1 of 3) 3.4.24.13 - IgA-specific metalloendopeptidase / Immunoglobulin A1 proteinase | 3'UTR | |
| Cre12.g503250 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTACACACATGAATACCGTCCGCCTTCCGGTCGTCTGTAACTGCTGC |
| Internal bar code: | GACTCAAGCAAAAGTAGATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4784 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCCTGTGTTTGGATTGG |
| Suggested primer 2: | CGTACGGGTACTGATGTCGG |