| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069819 |
| Chromosome: | chromosome 8 |
| Location: | 2045418 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g368900 | TSA2 | (1 of 1) PTHR11777//PTHR11777:SF14 - ALANYL-TRNA SYNTHETASE // ALANINE--TRNA LIGASE, CHLOROPLASTIC-RELATED; Alanyl-tRNA-synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCATGTGTGAGAGTCTGAGGGTGTGAGGGAGAGAGACACAAGAGGTACG |
| Internal bar code: | GGAACTCCAATCGGTTATAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGGGTGTTCATGC |
| Suggested primer 2: | TTCTTCTCATGGGGTTGCCC |