| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069824 |
| Chromosome: | chromosome 12 |
| Location: | 5901716 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533550 | PYK1 | (1 of 1) PTHR11817:SF5 - PYRUVATE KINASE-RELATED; Pyruvate kinase 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGATGTCAAGGCTGGCCAGGGCCTTGCAGGTTTAAACACTCGCGCGTCA |
| Internal bar code: | GTGTCTGATAATTTGTAGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1660 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTACACTCAAAGCAGCGA |
| Suggested primer 2: | GGACACGACCGATGAGATCC |