| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069828 |
| Chromosome: | chromosome 7 |
| Location: | 3394959 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335200 | GBA1,EFG12 | (1 of 1) PTHR23115//PTHR23115:SF165 - TRANSLATION FACTOR // TYPA-LIKE TRANSLATION ELONGATION FACTOR SVR3-RELATED; Putative chloroplast TypA translation elongation GTPase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACGCCAACGGACTTGCGCCACGCACATCTACGACATGTATGCATAAT |
| Internal bar code: | TTATGGTAGTAGTCGTTTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3030 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCTGTTAACGAGACGCC |
| Suggested primer 2: | AGCTGTCGGTGAGGAATGTG |