Insertion junction: CLIP2.069832_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.069832
Chromosome:chromosome 11
Location:358551
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467577 (1 of 2) PF00319 - SRF-type transcription factor (DNA-binding and dimerisation domain) (SRF-TF) gene_edge/mRNA_edge/5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGCGATCCCGCAAGCCCATCCCACCTGGCTCCGTGTGCACCTCCAAGT
Internal bar code:TGCGGCTCGTTCATGATTTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:712
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCAACGAGTCCCGAACC
Suggested primer 2:GCACAACGAGAGGTCAAGGA