| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069838 |
| Chromosome: | chromosome 7 |
| Location: | 5766298 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353300 | PXN1,MCP22 | Peroxisomal NAD+ carrier; (1 of 2) K13354 - solute carrier family 25 (peroxisomal adenine nucleotide transporter), member 17 (SLC25A17, PMP34) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGTTCCACCTTACGCCTGTGCCAGGCGAGTAGGCGCGGACCAAACAC |
| Internal bar code: | CAGTCGGGGTCCGGCTGAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2849 |
| LEAP-Seq percent confirming: | 98.8764 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGGGGTGGAATGACATT |
| Suggested primer 2: | TCCGGCTGAAGAGTTTGGTC |