| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.069852 |
| Chromosome: | chromosome 10 |
| Location: | 4069984 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448000 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTTAGGTACGTGAACCGATTGCGCCGTGGCAATCATCAAGTGCTACTG |
| Internal bar code: | CTGTGTCGGCGGTTTGTAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3728 |
| LEAP-Seq percent confirming: | 74.6667 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCATCACCACCTTTTTGT |
| Suggested primer 2: | TGGTCGCAGATGAACAGCTT |