| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.069855 |
| Chromosome: | chromosome 17 |
| Location: | 2217059 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAATCAAAAGGAAATGCTCCAGTATCACTGACGCCAACCTGTTCCCTAC |
| Internal bar code: | TTGTCTCTTGAGAGTGTCTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGTAGGGAAGCAGAGGG |
| Suggested primer 2: | CCTACCAGTGTGTTGGGCAT |