| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.069860 |
| Chromosome: | chromosome 12 |
| Location: | 7147867 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559555 | (1 of 4) 2.3.1.43 - Phosphatidylcholine--sterol O-acyltransferase / Phospholipid--cholesterol acyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGGGGCAAAAACGTGTCCACACGTGTTTCTCCACGCTCGCGTAGACT |
| Internal bar code: | CCCCGAGCGATGCACGAGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1456 |
| LEAP-Seq percent confirming: | 31.8182 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCTCCGAATCAGCTACC |
| Suggested primer 2: | GGAGTGGTCCAACCGGAAAT |