| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.069898 |
| Chromosome: | chromosome 16 |
| Location: | 7995563 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691888 | FBP3 | Fructose-2%252C6-bisphosphate 2-phosphatase; (1 of 2) 2.7.1.105 - 6-phosphofructo-2-kinase / Phosphofructokinase 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAACTTAATGGGGCGCACTCAGCTGAGCGGTGGCGTATCCATGCGGCG |
| Internal bar code: | GGCTGATTCTGAAGGTAAGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1340 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTGGGTGCATTTTGTGG |
| Suggested primer 2: | GTGAGGCAGGCAGTTGTTTG |