Insertion junction: CLIP2.069914_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.069914
Chromosome:chromosome 15
Location:1652316
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre19.g750697 OPR111,NCL37 Nuclear Control of chloroplast Like 37; (1 of 1) 2.4.99.3 - Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase / GalNAc alpha-2,6-sialyltransferase I 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTCGTATCTTGGTTTTGGGGTAATGTCTAGCTCCAGACAAAAACGGC
Internal bar code:ACCTTCGACCCAGTCTTCCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:684
LEAP-Seq percent confirming:21.7391
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACGGCTTGTCATTTGC
Suggested primer 2:AGGATTGCATTGGAAGGGGG