| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069919 |
| Chromosome: | chromosome 16 |
| Location: | 6007926 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678400 | (1 of 1) PTHR22847//PTHR22847:SF412 - WD40 REPEAT PROTEIN // TRANSDUCIN/WD40 DOMAIN-CONTAINING PROTEIN | 5'UTR | |
| Cre16.g678450 | Glycosyl transferase; (1 of 2) PTHR10859//PTHR10859:SF50 - GLYCOSYL TRANSFERASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCGTATACAATACATTAACGACACTGGTCTAGCTTTTGTTTACTTGG |
| Internal bar code: | TTATGCCTCAGTGATATATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2900 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGATCCCAAGCCACTTTCC |
| Suggested primer 2: | CACCGGGATAATGACGCTGA |