| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069920 |
| Chromosome: | chromosome 7 |
| Location: | 3623091 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337050 | (1 of 1) K13346 - peroxin-10 (PEX10) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGTGCAAGTGTTGTGCGAATGGGACGAGATGGGATGCGCAGGCAGGG |
| Internal bar code: | ATATTTGACATCAACGTAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3657 |
| LEAP-Seq percent confirming: | 96.2687 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGGGAAGTAGGCAGGTG |
| Suggested primer 2: | TTCTGACATCTCTGCGGCAG |