| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069944 |
| Chromosome: | chromosome 12 |
| Location: | 3513654 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCCCCCCTGTTCTCCATGTACCACCGAAAGCGCACAGCTCCCCCAAT |
| Internal bar code: | ACACTGATTTCTGCTCTGTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4932 |
| LEAP-Seq percent confirming: | 93.0 |
| LEAP-Seq n confirming: | 93 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTTCCAGGCAGAGTTCA |
| Suggested primer 2: | ATCCGTTAGTTCGACGCCTC |