| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.069945 |
| Chromosome: | chromosome 16 |
| Location: | 1137607 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649950 | HFO24,HFO40 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCGGGTGTAGAAGATGCCGGAAGCTCAGATTCTGGGAGTGAAGCCTC |
| Internal bar code: | TTTGTGTAAAACGGTGTGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1817 |
| LEAP-Seq percent confirming: | 86.4865 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTTGGGCATGATGGTCAC |
| Suggested primer 2: | CCGCGAAGTTCCCGATATCA |