| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069950 |
| Chromosome: | chromosome 12 |
| Location: | 4465036 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g520250 | PAF1 | (1 of 1) K15174 - RNA polymerase II-associated factor 1 (PAF1); RNA polymerase II-associated factor 1 | 5'UTR |
| Cre12.g520300 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATCGATTCCTTCGCTCGCTTCTTTCTGCCAGCGGTGTCAAGGCCGGC |
| Internal bar code: | TTTCGATATAAATGCGGCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4210 |
| LEAP-Seq percent confirming: | 46.2963 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCTGAGTTGAGTTGGCT |
| Suggested primer 2: | AGAGTGTCCATGCTTGGGTG |