| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069956 |
| Chromosome: | plastome |
| Location: | 122893 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802313 | rbcL,ChreCp049,2717040 | (1 of 1) K01601 - ribulose-bisphosphate carboxylase large chain (rbcL); RuBisCO large subunit | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAACGGTCAAGACTTGTTAAACCGTCAGTCCATACTGTAGTCCATGTA |
| Internal bar code: | TGGTTGCTCCTCGCACGACCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 8.62069 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTTATCCCCTGACAGGA |
| Suggested primer 2: | GCCAACGCATGAATGGTTGT |