| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.069957 |
| Chromosome: | chromosome 12 |
| Location: | 3964771 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515550 | VPS33 | SM/Sec1-family protein; (1 of 1) PTHR11679//PTHR11679:SF1 - VESICLE PROTEIN SORTING-ASSOCIATED // VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 33B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTGGCGGAGAACCGCGCGGCAGGGTTGTTGTGTGACCTGACTGAACA |
| Internal bar code: | CACATCGTCTTAAGCGGGAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1536 |
| LEAP-Seq percent confirming: | 98.5294 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGCAACGTCTTCCTTG |
| Suggested primer 2: | TCATGACACCGTTGAGCACA |