| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069962 |
| Chromosome: | chromosome 17 |
| Location: | 6001343 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g742550 | 3'UTR | ||
| Cre17.g802126 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAATTATCTGTACACGGCATTGCCGGTTGGGAGGATCAGCGGCCAGCA |
| Internal bar code: | ACCTTTGGGTAGGGTCTAGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2928 |
| LEAP-Seq percent confirming: | 77.907 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATAGATGTTGCACCTGC |
| Suggested primer 2: | TTCATTCCGGACCTCAGCAC |