| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069968 |
| Chromosome: | chromosome 10 |
| Location: | 3081239 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441250 | TMEM231 | (1 of 1) K19362 - transmembrane protein 231 (TMEM231); Transmembrane protein 231 | 3'UTR |
| Cre10.g441300 | (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAACAATGCTCTAGCTTCAGAGTAGTCCCGATGTAACTGTAAAGGTCC |
| Internal bar code: | TCCTGTCGGTTTAATCGCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3608 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGGACACCTCGGAAAGC |
| Suggested primer 2: | AGGCAGGTTGCTGAAGTCTC |