| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069977 |
| Chromosome: | chromosome 6 |
| Location: | 7033304 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800729 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGACATGCATTTCGTTGGTTCAATATTAATGTTCACTTTGGACTTTCT |
| Internal bar code: | GGGGGTGAGCAGAGGCTTGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 26.7857 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGAAGCCGAGCTGTTTG |
| Suggested primer 2: | CGTATTCGGAGTGGATGGGG |