Insertion junction: CLIP2.069996_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.069996
Chromosome:chromosome 13
Location:3227588
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g585500 HAP1 (1 of 12) IPR000326 - Phosphatidic acid phosphatase type 2/haloperoxidase; Putative haloperoxidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATTGTAATGCTTCCGTTCTTAGGTATACCATTGCATCCTCCTCCTCCTT
Internal bar code:GCATTGGTCGGGTGCCATGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:494
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCCGACACACACATACAA
Suggested primer 2:CCTGAGAGCATCGCGGTATT