| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070000 |
| Chromosome: | chromosome 15 |
| Location: | 5591188 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre26.g756947 | (1 of 1) PF15253 - SCL-interrupting locus protein N-terminus (STIL_N) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGAAGCTGCCGGGGAGCGGCTGAGGGCGCTGCGAGTGTCTGCCACG |
| Internal bar code: | ATGTAATTTTGCCTGTATTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 1.33333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAAGAATTCGGCAGGGG |
| Suggested primer 2: | CGGCGCTTCTTATCAACACG |