| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070005 |
| Chromosome: | chromosome 6 |
| Location: | 8343027 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307450 | SPL1 | (1 of 1) K12825 - splicing factor 3A subunit 1 (SF3A1, SAP114); SF3A1 splicing factor 3a, subunit 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGAGGCAGCGGGGCAGGGCACGCACGAGGCAGCGCTACAAACGGCGTT |
| Internal bar code: | TGCTATAGTAGGGCCAGCGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCTCAACACCTCTTCCT |
| Suggested primer 2: | TGGCCAATGAGCTTCTCGTT |