| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070021 |
| Chromosome: | chromosome 12 |
| Location: | 5260466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527800 | DNAL4,LC10,ODA-LC10,DLC10,DLL3,MOT24 | Outer Arm Dynein Light Chain; (1 of 1) K10412 - dynein light chain 4, axonemal (DNAL4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGAGGAGGGTAGGAAATCCGAAAAGTTGCCGGTGGCCTCTGGTTGTAA |
| Internal bar code: | AGGAGATTTGGGTAAAGCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3281 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCAGTGTGGCTGTGTA |
| Suggested primer 2: | AACGACGCAGAAAAGTGCAC |