Insertion junction: CLIP2.070027_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.070027
Chromosome:chromosome 15
Location:1392048
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g801734 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCACTAATGCGGTGCAAACGGAGGTGCGGTACTGCGAACGCGCTGCGC
Internal bar code:TCAACTCATGTCTTAAAGTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:119
LEAP-Seq percent confirming:1.53846
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:64
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGTGCCCACAAACTCACA
Suggested primer 2:GTCAGTTCTCCGGCCAAGAA