| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070029 |
| Chromosome: | chromosome 12 |
| Location: | 7923921 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553100 | (1 of 1) PF16529 - WD40 region of Ge1, enhancer of mRNA-decapping protein (Ge1_WD40) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTGGGAATGTGACAAGGAAAACGGGAGAGATCGGTTGGTGCGTGGA |
| Internal bar code: | TACGGAATCCATTCTTACTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTGCGGTACCGGTAAT |
| Suggested primer 2: | ATGAGGCCAATGACCTGGTG |