Insertion junction: CLIP2.070033_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.070033
Chromosome:chromosome 12
Location:5260000
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g527800 DNAL4,LC10,ODA-LC10,DLC10,DLL3,MOT24 Outer Arm Dynein Light Chain; (1 of 1) K10412 - dynein light chain 4, axonemal (DNAL4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTGCTGAAGTTGCGACATTAATGGTCAAGGCTTCCTTCTTTTGGGGCTT
Internal bar code:TGGAACTTTTAGTCGAATGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2860
LEAP-Seq percent confirming:97.4026
LEAP-Seq n confirming:75
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:77

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGCCGAGTTTAGATTGCG
Suggested primer 2:GCGCTCGCAATCTAACTGTG