| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.070034 |
| Chromosome: | chromosome 1 |
| Location: | 1463915 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007811 | (1 of 1) PF15383 - Transmembrane protein 237 (TMEM237) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGTGTGGCTCCACGGTGTGGCTCCTTGGCGGCGCAATCGTTGTGAA |
| Internal bar code: | TCAACCATGAAGTACGTCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1634 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGCAGGGATGAGCCTTTC |
| Suggested primer 2: | CACATACCGTCGCTTTGCTG |