| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.070035 |
| Chromosome: | chromosome 3 |
| Location: | 3800749 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169850 | TSC1 | (1 of 1) PTHR10890:SF3 - CYSTEINE--TRNA LIGASE, CYTOPLASMIC; Cysteinyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGAAGGAGCCCAATCATCTGACGGACGCAGAGGGTTGGGACAGGGGT |
| Internal bar code: | TTAATACCCATGTTTGGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4250 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACTTTCGCCATGACCGT |
| Suggested primer 2: | TCACGCTCCGGCTTAGATTC |