| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070048 |
| Chromosome: | chromosome 3 |
| Location: | 5783334 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187650 | (1 of 5) PF04884 - Vitamin B6 photo-protection and homoeostasis (DUF647) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGAACCACTTCTTTAAAGGAGTGACAGCGCAGAGCTTCCCTGCAGTAT |
| Internal bar code: | CGGGGTTCGGATCTATGTGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2826 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGCGGTGACCATACAT |
| Suggested primer 2: | ATGAAGGCACATCAGCGACA |