| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.070060 |
| Chromosome: | chromosome 2 |
| Location: | 6843855 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388393 | ELG13 | (1 of 39) PTHR11062//PTHR11062:SF21 - EXOSTOSIN HEPARAN SULFATE GLYCOSYLTRANSFERASE -RELATED // SUBFAMILY NOT NAMED; Exostosin-like glycosyltransferase 13 | 3'UTR |
| Cre09.g388430 | (1 of 2) IPR000104//IPR001202 - Antifreeze protein, type I // WW domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATCAAAGGTCAAAGGAATTTGGGTTTCTCCCACAAGTGGTGGGCTGA |
| Internal bar code: | CTTTGTTACTAGCCCACATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3750 |
| LEAP-Seq percent confirming: | 91.9355 |
| LEAP-Seq n confirming: | 57 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGGCGCAATCAGTAGTAT |
| Suggested primer 2: | TGGCCTACAACTACACGCTG |