| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070067 |
| Chromosome: | chromosome 1 |
| Location: | 5365345 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037900 | (1 of 2) K03257 - translation initiation factor 4A (EIF4A) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGCGCTGTGTGTGCAGGAAGCTTGCGGGAACGAGGCGGGCTGGATGC |
| Internal bar code: | GGGGCGCATCGTTATAATCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 68.2927 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGCAACATGTACGATGTC |
| Suggested primer 2: | GCCATTGCGATTGGTGTTGT |