| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070075 |
| Chromosome: | chromosome 2 |
| Location: | 8483235 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141950 | MOT19 | (1 of 5) PF03765 - CRAL/TRIO, N-terminal domain (CRAL_TRIO_N); Predicted protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCGAGGGGTTTGGAGGGGTGGGGTTGAGCACTTGCTCCCCCCCCCGGC |
| Internal bar code: | TGTAACACCTACCAGCGGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 702 |
| LEAP-Seq percent confirming: | 76.5957 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTCAAGCCGGGCATGAT |
| Suggested primer 2: | ATTGTGGCACTGCGTTGTTC |